deepvent exo dna polymerase (New England Biolabs)
New England Biolabs is a verified supplier 
New England Biolabs manufactures this product 
95
Structured Review
New England Biolabs
deepvent exo dna polymerase
Deepvent Exo Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deepvent+dna+polymerase/Deep+Vent+(exo/us12553044-608-22-25
Average 95 stars, based on 334 article reviews
Deepvent Exo Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deepvent+dna+polymerase/Deep+Vent+(exo/us12553044-608-22-25
Average 95 stars, based on 334 article reviews
deepvent exo dna polymerase - by Bioz Stars,
2026-09
95/100 stars
Images
Related Articles
Expressing:Article Title: Mitochondria-targeted oligomeric α-synuclein induces TOM40 degradation and mitochondrial dysfunction in Parkinson's disease and parkinsonism-dementia of Guam. Article Snippet: NPSCs were derived from both the control and SNCA-Tri iPSC lines using PSC neural induction medium (Thermo Fisher A1647801, USA) following the manufacturer’s instructions and the protocol explained earlier [11]. .. Construction of pCW WT-α-Syn-Flag Expression Vector: The doxycycline (Dox) inducible mammalian pCW WT-α-Syn-Flag expression vector was created by inserting the full-length α-Syn, amplified from the pcDNA WT-αSyn plasmid using Article Title: Mitochondria-Targeted Oligomeric α-Synuclein Induces TOM40 Degradation and Mitochondrial Dysfunction in Parkinson’s Disease and Parkinsonism-Dementia of Guam Article Snippet: Neural progenitor stem cells (NPSCs) were derived from both the control and SNCA-tri iPSC lines using PSC neural induction medium (Thermo Fisher A1647801, USA) following the manufacturer’s instructions and the protocol explained earlier 11. .. Plasmid constructs Construction of pCW WT-α-Syn-Flag Expression Vector: The doxycycline (Dox) inducible mammalian pCW WT-α-Syn-Flag expression vector was created by inserting the full-length α-Syn, ampli ed from the pcDNA WT-α-Syn plasmid using Article Title: Mitochondria-targeted oligomeric α-synuclein induces TOM40 degradation and mitochondrial dysfunction in Parkinson’s disease and parkinsonism-dementia of Guam Article Snippet: NPSCs were derived from both the control and SNCA-Tri iPSC lines using PSC neural induction medium (Thermo Fisher A1647801, USA) following the manufacturer’s instructions and the protocol explained earlier [ ]. .. Construction of pCW WT-α-Syn-Flag Expression Vector: The doxycycline (Dox) inducible mammalian pCW WT-α-Syn-Flag expression vector was created by inserting the full-length α-Syn, amplified from the pcDNA WT-α-Syn plasmid using Plasmid Preparation:Article Title: Mitochondria-targeted oligomeric α-synuclein induces TOM40 degradation and mitochondrial dysfunction in Parkinson's disease and parkinsonism-dementia of Guam. Article Snippet: NPSCs were derived from both the control and SNCA-Tri iPSC lines using PSC neural induction medium (Thermo Fisher A1647801, USA) following the manufacturer’s instructions and the protocol explained earlier [11]. .. Construction of pCW WT-α-Syn-Flag Expression Vector: The doxycycline (Dox) inducible mammalian pCW WT-α-Syn-Flag expression vector was created by inserting the full-length α-Syn, amplified from the pcDNA WT-αSyn plasmid using Article Title: Mitochondria-Targeted Oligomeric α-Synuclein Induces TOM40 Degradation and Mitochondrial Dysfunction in Parkinson’s Disease and Parkinsonism-Dementia of Guam Article Snippet: Neural progenitor stem cells (NPSCs) were derived from both the control and SNCA-tri iPSC lines using PSC neural induction medium (Thermo Fisher A1647801, USA) following the manufacturer’s instructions and the protocol explained earlier 11. .. Plasmid constructs Construction of pCW WT-α-Syn-Flag Expression Vector: The doxycycline (Dox) inducible mammalian pCW WT-α-Syn-Flag expression vector was created by inserting the full-length α-Syn, ampli ed from the pcDNA WT-α-Syn plasmid using Article Title: Mitochondria-targeted oligomeric α-synuclein induces TOM40 degradation and mitochondrial dysfunction in Parkinson’s disease and parkinsonism-dementia of Guam Article Snippet: NPSCs were derived from both the control and SNCA-Tri iPSC lines using PSC neural induction medium (Thermo Fisher A1647801, USA) following the manufacturer’s instructions and the protocol explained earlier [ ]. .. Construction of pCW WT-α-Syn-Flag Expression Vector: The doxycycline (Dox) inducible mammalian pCW WT-α-Syn-Flag expression vector was created by inserting the full-length α-Syn, amplified from the pcDNA WT-α-Syn plasmid using Article Title: Optimization of Replication, Transcription, and Translation in a Semi-Synthetic Organism Article Snippet: PCR amplifications were performed in a CFX Connect Real-Time PCR Detection System (BioRad). .. Plasmid minipreps, or Golden Gate assembled plasmids (0.5 μL to 2 μL, 0.5 ng/μL to 5 ng/μL), or dNaM-containing oligonucleotides (0.025 ng) were PCR amplified in total reaction volumes of 15 μL under the following conditions: OneTaq Standard Reaction Buffer (1×, New England BioLabs), dNTPs (400 μM), SYBR Green I (1×, Life Technologies), MgSO4 (2.2 mM), primers P3-4 or P5-6 (1 μM each, see Table S4 for primer sequences), d5SICSTP (65 μM), dMMO2BIOTP (65 μM; structure shown in Figure S6), OneTaq DNA polymerase (0.27 U, New England BioLabs), Amplification:Article Title: Mitochondria-targeted oligomeric α-synuclein induces TOM40 degradation and mitochondrial dysfunction in Parkinson's disease and parkinsonism-dementia of Guam. Article Snippet: NPSCs were derived from both the control and SNCA-Tri iPSC lines using PSC neural induction medium (Thermo Fisher A1647801, USA) following the manufacturer’s instructions and the protocol explained earlier [11]. .. Construction of pCW WT-α-Syn-Flag Expression Vector: The doxycycline (Dox) inducible mammalian pCW WT-α-Syn-Flag expression vector was created by inserting the full-length α-Syn, amplified from the pcDNA WT-αSyn plasmid using Article Title: Mitochondria-targeted oligomeric α-synuclein induces TOM40 degradation and mitochondrial dysfunction in Parkinson’s disease and parkinsonism-dementia of Guam Article Snippet: NPSCs were derived from both the control and SNCA-Tri iPSC lines using PSC neural induction medium (Thermo Fisher A1647801, USA) following the manufacturer’s instructions and the protocol explained earlier [ ]. .. Construction of pCW WT-α-Syn-Flag Expression Vector: The doxycycline (Dox) inducible mammalian pCW WT-α-Syn-Flag expression vector was created by inserting the full-length α-Syn, amplified from the pcDNA WT-α-Syn plasmid using Article Title: Supporting Information PCR with an Expanded Genetic Alphabet Article Snippet: After quenching with EDTA to final concentration of 10 mM, the dsDNA templates were purified using a 1.8% agarose gel, quantified by fluorescent dye binding (Quant-iT dsDNA HS Assay kit, Invitrogen), sequenced (see Figures S5-S10), and used for PCR. .. PCR amplification of template D1 (1 ng) was carried out in 1 ThermoPol Reaction Buffer (New England Biolabs; 20 mM Tris-HCl buffer (pH 8.8), 10 mM KCl, 10 mM (NH4)2SO4, 4 mM MgSO4, 0.1% Triton X-100), 0.8 mM each natural dNTP, 1 μM each primer1 and primer2 and 0.03 unit/μL Article Title: Optimization of Replication, Transcription, and Translation in a Semi-Synthetic Organism Article Snippet: PCR amplifications were performed in a CFX Connect Real-Time PCR Detection System (BioRad). .. Plasmid minipreps, or Golden Gate assembled plasmids (0.5 μL to 2 μL, 0.5 ng/μL to 5 ng/μL), or dNaM-containing oligonucleotides (0.025 ng) were PCR amplified in total reaction volumes of 15 μL under the following conditions: OneTaq Standard Reaction Buffer (1×, New England BioLabs), dNTPs (400 μM), SYBR Green I (1×, Life Technologies), MgSO4 (2.2 mM), primers P3-4 or P5-6 (1 μM each, see Table S4 for primer sequences), d5SICSTP (65 μM), dMMO2BIOTP (65 μM; structure shown in Figure S6), OneTaq DNA polymerase (0.27 U, New England BioLabs), Construct:Article Title: Mitochondria-Targeted Oligomeric α-Synuclein Induces TOM40 Degradation and Mitochondrial Dysfunction in Parkinson’s Disease and Parkinsonism-Dementia of Guam Article Snippet: Neural progenitor stem cells (NPSCs) were derived from both the control and SNCA-tri iPSC lines using PSC neural induction medium (Thermo Fisher A1647801, USA) following the manufacturer’s instructions and the protocol explained earlier 11. .. Plasmid constructs Construction of pCW WT-α-Syn-Flag Expression Vector: The doxycycline (Dox) inducible mammalian pCW WT-α-Syn-Flag expression vector was created by inserting the full-length α-Syn, ampli ed from the pcDNA WT-α-Syn plasmid using other:Article Title: Chemically-enhanced primer compositions, methods and kits Article Snippet: Non-limiting examples of commercially available polymerases that can be used in the methods described herein include, but are not limited to, TaqFS®, AmpliTaq® CS (Applied Biosystems), AmpliTaq FS (Applied Biosystems), AmpliTaq Gold ° (Applied Biosystems), Kentaq1 (AB Peptide, St. Louis, Mo.), Taquenase (ScienTech Corp., St. Louis, Mo.), ThermoSequenase (Amersham), Bst polymerase, VentR(exo−) DNA polymerase, ReaderTMTaq DNA polymerase, Polymerase Chain Reaction:Article Title: Supporting Information PCR with an Expanded Genetic Alphabet Article Snippet: After quenching with EDTA to final concentration of 10 mM, the dsDNA templates were purified using a 1.8% agarose gel, quantified by fluorescent dye binding (Quant-iT dsDNA HS Assay kit, Invitrogen), sequenced (see Figures S5-S10), and used for PCR. .. PCR amplification of template D1 (1 ng) was carried out in 1 ThermoPol Reaction Buffer (New England Biolabs; 20 mM Tris-HCl buffer (pH 8.8), 10 mM KCl, 10 mM (NH4)2SO4, 4 mM MgSO4, 0.1% Triton X-100), 0.8 mM each natural dNTP, 1 μM each primer1 and primer2 and 0.03 unit/μL Article Title: Optimization of Replication, Transcription, and Translation in a Semi-Synthetic Organism Article Snippet: PCR amplifications were performed in a CFX Connect Real-Time PCR Detection System (BioRad). .. Plasmid minipreps, or Golden Gate assembled plasmids (0.5 μL to 2 μL, 0.5 ng/μL to 5 ng/μL), or dNaM-containing oligonucleotides (0.025 ng) were PCR amplified in total reaction volumes of 15 μL under the following conditions: OneTaq Standard Reaction Buffer (1×, New England BioLabs), dNTPs (400 μM), SYBR Green I (1×, Life Technologies), MgSO4 (2.2 mM), primers P3-4 or P5-6 (1 μM each, see Table S4 for primer sequences), d5SICSTP (65 μM), dMMO2BIOTP (65 μM; structure shown in Figure S6), OneTaq DNA polymerase (0.27 U, New England BioLabs), SYBR Green Assay:Article Title: Optimization of Replication, Transcription, and Translation in a Semi-Synthetic Organism Article Snippet: PCR amplifications were performed in a CFX Connect Real-Time PCR Detection System (BioRad). .. Plasmid minipreps, or Golden Gate assembled plasmids (0.5 μL to 2 μL, 0.5 ng/μL to 5 ng/μL), or dNaM-containing oligonucleotides (0.025 ng) were PCR amplified in total reaction volumes of 15 μL under the following conditions: OneTaq Standard Reaction Buffer (1×, New England BioLabs), dNTPs (400 μM), SYBR Green I (1×, Life Technologies), MgSO4 (2.2 mM), primers P3-4 or P5-6 (1 μM each, see Table S4 for primer sequences), d5SICSTP (65 μM), dMMO2BIOTP (65 μM; structure shown in Figure S6), OneTaq DNA polymerase (0.27 U, New England BioLabs), |